» Methods
We are using various methods:
(* measurements for collaboration partners or companies possible!)
Chromatography:
AF4*: Asymmetric Field Flow Fractionation incl. DLS-Detector
FPLC*: Purification of proteins
HPLC*: Analytical liquid chromatography
Fermentation:
Automated Fermenter*: E. coli – 2,5L, 5L, 30L | Picchia pastoris – 2,5L
Cell break-up*: Crossflow, homogenisator, ball mill
Microscopy
AFM: Atomic force microscopy
Light microscopy* | Fluorescence microscopy* | Confocal microscopy* | TIRF microscopy*
SEM*: Low-Vacuum Scanning electron microscopy incl. SerialBlockface-Imaging (SBF)
SEM/ESEM: Scanning electron microscopy incl. cryo stage, EDX, WDX
TEM: Transmission electron microscopy incl. cryo stage
Physico-chemical analysis
DSC*: Differential scanning calorimetry
DMTA*: Dynamic mechanical thermoanalysis
Surface characterization: BET method, profilometer
Permeation measurements
Universal test machine for mN-loads*: Static/dynamic tension and compression
Microfluidics*
Nanoindenter test
Rheology*
TGA*: Thermogravimetric analysis
Probe treatment and processing
Organic synthesis*
Spinning methods*: Electro-spinning, wet-spinning, biomimetic spinning
3D-Printing*: Bioprinting
Coating methods*: Dip coating, thin-film coating
Film casting*
Molecular biology
Host engineering*
Molecular cloning*
Screening*: Genes, proteins
Sequencing
Cell culture*
Spectroscopy:
CD-Spectroscopy*
Fluorescence Spectroskopy*
FT-IR*: Reflexion and transmission microscope, ATR, Fluid cell, polarisation
Laser diffraction spectrometry
Mass spectrometry
NMR
Raman spectroscopy
SAXS, WAXS, Synchrotron: X-ray diffraction analysis
UV-VIS spectroscopy*